组会讲课人员:王婷
Analysis of single extracellular vesicles for biomedical applications with especial emphasis on cancer investigations
单个细胞外囊泡在生物医学应用的的分析,特别强调癌症的评估
主讲人:王婷
Trends in Analytical Chemistry | July 2022 | Volume 152 | 116604 | DOI:10.1016/j.trac.2022.116604
Abstract:
Extracellular vesicles (EVs) are lipid membrane enclosed nano-sized vesicles that are secreted by all known organisms. These vesicles are increasingly recognized as important circulating biomarkers for the diagnosis and prognosis of different diseases including various types of cancer, owing to their essential role in intercellular communication. EVs preserve heterogeneity in both physical properties and cargos, which makes it extraordinarily tough to fully exploit their clinical potential. Therefore, comprehensive characterization of single EVs and their sensitive detection are urgently demanded. In this article, we survey the latest progress in single EVs analysis with innovative discoveries in heterogeneity and highlight the various label-free and labelling approaches of single EVs detection. Furthermore, the stateof-the-art advances in single EV-detection based biomedical applications with especial emphasis on cancer investigations are summarized. To the end, the challenges and prospects for exploiting new
system in the field of single EVs study are discussed.
摘要:
细胞外囊泡(EVs)是由所有已知生物分泌的脂质膜包裹的纳米级囊泡。由于其在细胞间通讯中的重要作用,这些囊泡越来越被认为是包括各种类型癌症在内的不同疾病的诊断和预后的重要循环生物标志物。EV在物理性质和物质上都保持了异质性,这使得充分开发其临床潜力变得异常困难。因此,迫切需要对单个EV进行全面的表征及其灵敏的检测。本文综述了单个EV检测的最新进展和异质性方面的创新发现,并重点介绍了单个EV检测的各种非标记和标记方法。此外,总结了基于单一EV检测的生物医学应用特别是癌症研究的最新进展。最后,讨论了单个EV研究领域开发新系统面临的挑战和前景。