组会讲课人员:胥宁格
Design of Activatable NIR-II Molecular Probe for In Vivo Elucidation of Disease-Related Viscosity Variations
可激活NIR-II分子探针的设计,用于疾病相关黏度变化的活体检测
主讲人:胥宁格
Anal. Chem | 2020, 92, 4177−4181 | DOI:10.1021/acs.analchem.0c00634
Abstract:
A clear elucidation of a disease-related viscosity change in vivo is significant yet highly challenging as well. Fluorescence imaging in the second near infrared region (NIR-II, 1000−1700 nm) has gained increasing attention for observation in living organisms, but a viscosity-activatable fluorescent probe emitting at this region remains a vacancy. Herein, we report the first panel of a viscosity-activated NIR-II emissive fluorescent probe WD-X. By embedding different substituents into the WD-X platform and screening, we obtained an ideal probe, WD-NO2, which displayed the best combination of properties, including a 31-fold fluorescence enhancement in response to viscosity, insensitivity to environments (pH, polarity), and relatively high quantum yield (1.6% in glycerol). WD-NO2 was successfully applied to track the variation of viscosity in diabetes-induced liver injury in vivo.
摘要:
明确阐明与疾病相关的体内黏度变化是重要的,但也极具挑战性。近红外二区(NIR-II, 1000 ~ 1700 nm)的荧光成像在活体生物的观察中得到了越来越多的关注,但在该区域发射的粘度激活荧光探针仍然是一个空缺。在此,作者报道了一个粘度激活NIR-II发射荧光探针WD-X。通过将不同的取代基嵌入WD-X平台并进行筛选,作者得到了一个理想的探针WD-NO2,它具有总的最佳性能,包括对粘度的荧光增强31倍,对环境(pH、极性)不敏感,以及相对较高的量子产率(在甘油中1.6%)。WD-NO2被成功的应用于在体内追踪糖尿病肝损伤中黏度的变化。